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Evidence Guide: MSL973016 - Perform aseptic techniques

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL973016 - Perform aseptic techniques

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare for aseptic sampling or transfer

  1. Check the sampling procedure conforms with the required sampling plan
Check the sampling procedure conforms with the required sampling plan

Completed
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Teacher:
Evidence:

 

 

 

 

 

 

 

Use specified personal protective equipment (PPE)

Completed
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Prepare the work area for safe and effective sample transfer

Completed
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Select equipment and materials specified by the procedure

Completed
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Evidence:

 

 

 

 

 

 

 

Organise equipment to minimise contamination during manipulations

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Label containers for clear identification

Completed
Date:

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Record details in relevant log or database

Completed
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Evidence:

 

 

 

 

 

 

 

Macroscopically assess media and/or reagents to confirm sterility before use

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Transfer materials aseptically

  1. Protect the integrity of the sample source
Protect the integrity of the sample source

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Sterilise inoculating loops and/or pipette where used to prevent contamination

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Perform transfer while minimising opportunities for contamination and cross-infection

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Protect the integrity of the sample source and destination after transfer, and before sealing the transport or culture vessel

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Re-sterilise inoculating loops, minimising the generation of aerosols as required

Completed
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Evidence:

 

 

 

 

 

 

 

Perform quality control checks to confirm aseptic transfer was successful

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Label transport or culture vessels for clear identification

Completed
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Evidence:

 

 

 

 

 

 

 

Maintain work area and equipment to prevent cross-infection and contamination

  1. Place disposable and reusable items into relevant receptacles
Place disposable and reusable items into relevant receptacles

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Clean and disinfect work area and equipment after use

Completed
Date:

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Evidence:

 

 

 

 

 

 

 

Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Follow personal hygiene procedures

Completed
Date:

Teacher:
Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge